MARIS STELLA COLLEGE
(AUTONOMOUS), VIJAYAWADA – 8
(Affiliated to Krishna
University, Machilipatnam)
SYLLABUS
Subject: Botany
Semester: I
Course
Title: Fundamentals of
Microbes and Course Code: 20BTCCMN14
Nonvascular Plants
No. of Hours: 60 LTP:
402 Credits: 4
Objectives:
- To Understand the Structure, Reproduction, Classification and
Economic importance of Microbes, Algae, Fungi and Bryophytes.
- To provide hands on training for cultivation of mushrooms.
- To demonstrate the effectiveness of the
biofertilizer in agriculture.
Course Outcomes:
On successful
completion of this course, the students will be able to:
CO1. Explain
origin of life on the earth.
CO-2.
Illustrate diversity among the viruses and prokaryotic organisms and can
categorize them.
CO-3. Classify
fungi, lichens, algae and bryophytes based on their structure, reproduction and
life cycles.
CO-4. Able to
understand prospects and cultivation of edible mushrooms.
CO-5.
Distinguish the use of biofertilizers and chemical fertilizers.
Elaborate the general characters and
evolutionary aspects in bryophyta.
CO-6. Analyze
and ascertain the plant disease symptoms due to viruses, bacteria and fungi.
CO-7. Recall
and explain the evolutionary trends among amphibians of the plant kingdom for
their shift to land habitat.
CO-8. Evaluate the ecological and economic value
of microbes, thallophytes and bryophytes.
UNIT – I: ORIGIN OF LIFE AND VIRUSES (12Hrs)
1. Origin of life, concept of primary
Abiogenesis; Five kingdom classification of R.H. Whittaker
2. Discovery of microorganisms, Pasteur
experiments, germ theory of diseases.
3. Shape and symmetry of viruses; structure of
TMV, Multiplication of TMV; A brief account of Prions and Viroids.
4. A general account on symptoms of plant
diseases caused by Viruses. Transmission of plant viruses and their control.
5. Significance of viruses in vaccine
production, bio-pesticides and as cloning vectors.
UNIT – II: SPECIAL GROUPS OF BACTERIA AND
EUBACTERIA (12 Hrs).
1. Brief account of Archaebacteria and
Actinomycetes.
2. Cell structure and nutrition of Eubacteria, Reproduction-
Asexual (Binary fission and endospores) and bacterial recombination
(Conjugation, Transformation, Transduction).
3. Economic importance of Bacteria with
reference to their role in Agriculture and industry (fermentation and
medicine).
4. A general account on symptoms of plant
diseases caused by Bacteria; Citrus canker.
5. Biofertilizers:
General account of the microbes used as biofertilizers, Rhizobium-Isolation,
Identification and mass multiplication. Cyanobacteria, Azolla, Anabaena
association and role of Azolla in agriculture.
UNIT – III: FUNGI &
LICHENS (12 Hrs)
1. General characteristics of fungi and
Ainsworth classification (Upto classes).
2. Structure, reproduction and life history of
(a) Rhizopus (Zygomycota) and (b) Puccinia (Basidiomycota).
3. Economic uses of fungi in food industry,
pharmacy and agriculture.
4. A general account on symptoms of plant
diseases caused by Fungi; Blast of Rice.
5. Lichens- structure and reproduction;
ecological and economic importance.
UNIT – IV: ALGAE (12 Hrs)
1. General characteristics of Algae (pigments,
flagella and reserve food material); Fritsch classification (Upto classes),
Thallus organization in Algae.
2. Occurrence, structure, reproduction and life
cycle of (a) Spirogyra (Chlorophyceae) and (b) Polysiphonia (Rhodophyceae).
3. Algal blooms, eutrophication and
biomagnification (From local water bodies). Algae as food, Medicine, feed,
biofuel and indicators of pollution.
UNIT – V: BRYOPHYTES (12
Hrs)
1. General
characteristics of Bryophytes; classification upto classes.
2. Occurrence,
morphology, anatomy, reproduction (developmental details are not needed) and
life cycle of (a) Marchantia (Hepaticopsida) and (b) Funaria (Bryopsida).
General account on evolution of sporophytes in Bryophyta.
Prescribed Text books:
●
Botany – I (Vriksha
Sastram-I) : Telugu Akademi, Hyderabad.
●
Pandey, B.P. (2013) College Botany, Volume-I, S. Chand Publishing,
New Delhi.
●
Hait, G., K. Bhattacharya & A.K. Ghosh (2011) A Text Book of
Botany, Volume-I, New Central Book Agency Pvt. Ltd., Kolkata.
●
Bhattacharjee, R.N., (2017) Introduction to Microbiology and
Microbial Diversity, Kalyani Publishers, New Delhi.
Books for Reference:
●
Dubey,
R.C. & D.K. Maheswari (2013) A TextBook of Microbiology, S. Chand & Company Ltd., New Delhi.
●
Pelczar
Jr., M.J., E.C.N. Chan & N.R. Krieg (2001) Microbiology, Tata Mc Graw Hill
Co, New Delhi.
●
Presscott,
L. Harley, J. and Klein, D. (2005) Microbiology, 6th edition, Tata McGraw –Hill
Co. New Delhi.
●
Alexopoulos,
C.J., C. W. Mims & M. Blackwell (2007) Introductory Mycology, Wiley&
Sons, Inc., New York.
●
Mehrotra,
R.S. & K. R. Aneja (1990) An Introduction to Mycology. New Age
International Publishers, New Delhi.
●
Kevin
Kavanagh (2005) Fungi; Biology and Applications John Wiley & Sons, Ltd.,
West Sussex, England.
●
John
Webster & R. W. S. Weber (2007) Introduction to Fungi, Cambridge University
Press, New York.
●
Fritsch,
F.E. (1945) The Structure & Reproduction of Algae (Vol. I & Vol. II)
Cambridge University Press Cambridge, U.K.
●
Bold,
H.C. & M. J. Wynne (1984) Introduction to the Algae, Prentice-Hall Inc.,
New Jersey.
●
Robert
Edward Lee (2008) Phycology. Cambridge University Press, New York.
●
Van
Den Hoek, C., D.G. Mann & H.M. Jahns (1996) Algae: An Introduction to
Phycology. Cambridge University Press, New York.
●
Shaw,
A.J.&B. Goffinet (2000) Bryophyte Biology. Cambridge University Press, New
York.
MARIS STELLA COLLEGE
(AUTONOMOUS), VIJAYAWADA – 8
(Affiliated to Krishna
University, Machilipatnam)
PRACTICAL
SYLLABUS
Subject: Botany
Semester: I
Course Title:
Fundamentals of Microbes
and
Nonvascular Plants Course Code: 20BTP111
No.
of Hours: 30 Credits:
1
Course Outcomes:
On successful
completion of this practical course, student shall be able to;
1. Demonstrate the techniques of use of lab equipment, preparing slides
and identify the material and draw diagrams exactly as it appears.
2. Observe and identify microbes and lower groups of plants on their
own.
3. Demonstrate the techniques of inoculation, preparation of media etc.
4. Identify the material in the permanent slides etc.
Experiment details:
1. Knowledge of
Microbiology laboratory practices and safety rules.
2. Knowledge of different equipment for
Microbiology laboratory (Spirit lamp, Inoculation loop, Hot-air oven,
Autoclave/Pressure cooker, Laminar air flow chamber and Incubator) and their
working principles.
3.
Demonstration of Gram’s staining technique for Bacteria.
4. Study of
Viruses (Corona, Gemini and TMV) using electron micrographs/ models.
5. Study of
Archaebacteria and Actinomycetes using permanent slides/ electron
micrographs/diagrams.
6. Study of
Anabaena and Oscillatoria using permanent/temporary slides.
7. Study of
different bacteria (Cocci, Bacillus, Vibrio and Spirillum) using permanent or
temporary slides/ electron micrographs/ diagrams.
8. Study/
microscopic observation of vegetative, sectional/anatomical and reproductive
structures of the following using temporary or permanent slides/ specimens/
mounts:
a. Fungi: Rhizopus and Puccinia
b. Lichens: Crustose, foliose and fruticose
c. Algae, Spirogyra and Polysiphonia
d. Bryophyta: Marchantia and Funaria (Slides)
9. Study of
specimens of Tobacco mosaic disease, Citrus canker and Blast of Rice.
10. Visit to
biofertilizer units in proximity.
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